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PNA Bio Inc
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Thermo Fisher
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Image Search Results
Journal: Nucleic Acids Research
Article Title: Visualization and quantitative analysis of extrachromosomal telomere-repeat DNA in individual human cells by Halo-FISH
doi: 10.1093/nar/gkv091
Figure Lengend Snippet: Representative images of human cells subjected to the Halo-FISH protocol. HeLa1.2.11 telomerase-positive cell ( A ) and GM847 ALT cell ( B ) are PNA probed for centromere DNA (orange), G-strand telomere-repeat DNA (green), C-strand telomere-repeat DNA (red) and counterstained with DAPI (blue). A set of 40 z-stack images were captured for each deproteinized nucleus at 0.2 μm intervals and were subjected to iterative deconvolution. TEL+: telomerase-positive, ALT+: ALT-positive. Scale bar, 10 μm.
Article Snippet: Peptide nucleic acid (PNA) probes used in this study are
Techniques:
Journal: Nucleic Acids Research
Article Title: Visualization and quantitative analysis of extrachromosomal telomere-repeat DNA in individual human cells by Halo-FISH
doi: 10.1093/nar/gkv091
Figure Lengend Snippet: Analysis of the number of ECTR DNA molecules detected in human primary, telomerase-positive and ALT cells. The number of G-strand ECTR DNA foci per nucleus ( A ), the number of C-strand ECTR DNA foci per nucleus ( B ) and the number of centromere DNA foci per nucleus ( C ) located in the Halo of the deproteinized nuclei are plotted for WI-38 primary cell, HT1080 and HeLa1.2.11 telomerase-positive cells, and GM847, VA13 and U2OS ALT cells. ( D ) GM847 deproteinized nucleus counterstained with DAPI (blue) to visualize the nuclear core and probed for G-strand (green) and C-strand (red) telomere-repeat DNA. White arrows through the merged image indicate the direction of the intensity linescan plots. The intensity linescan plots show colocalization patterns between G- and C-strand telomere-repeat DNA signals within the (a) nuclear core and the (b) Halo of the deproteinized nucleus. Scale bar, 10μm. ( E ) G- or C-strand telomere to centromere DNA foci ratio per nucleus within the nuclear core of the deproteinized nuclei for GM847, VA13 and U2OS ALT cells. 1°: primary, TEL+: telomerase-positive, ALT+: ALT-positive. Median and error bars representing the interquartile range are displayed.
Article Snippet: Peptide nucleic acid (PNA) probes used in this study are
Techniques:
Journal: Nucleic Acids Research
Article Title: Visualization and quantitative analysis of extrachromosomal telomere-repeat DNA in individual human cells by Halo-FISH
doi: 10.1093/nar/gkv091
Figure Lengend Snippet: Analysis of ECTR DNA content in human ALT cells. ( A ) Total G- and C-strand ECTR DNA content per nucleus displayed for GM847, VA13 and U2OS ALT cells. ( B ) Correlation analysis, per nucleus, between the total G- and C-strand ECTR DNA content and the number of nuclear core-localized centromere DNA foci in GM847, VA13 and U2OS ALT cells. A linear regression slope with a confidence level of 95% is displayed with each plot. ( C ) Percentage of total telomere-repeat DNA, per nucleus, attributed to G- or C-strand ECTR DNA in GM847, VA13 and U2OS ALT cells. Median and error bars representing the interquartile range are displayed.
Article Snippet: Peptide nucleic acid (PNA) probes used in this study are
Techniques: